Tuesday, December 31, 2019
Dreams And Visions Is Jesus Awakening The Muslim World
For six years, Ibrahim had been unconvincingly told by Muslim religious leaders in his city that the appearance of a glorious man in his dream was surely the Prophet Muhammad. When Ibrahim decided to share his dream with a Christian missionary in his town, he was overjoyed to find that the man who had come to him in his dream was not the Prophet Muhammad, but the same man found and described in Revelation 1:12-16. Before the encounter with the Christian missionary, Ibrahim had never heard of, much less read from the Bible. The discussion of his dream with the missionary ultimately led to a proclamation of the Gospel, in which Ibrahim would hear the Gospel for the first time, and ultimately come to faith in it weeks later. Stories ofâ⬠¦show more contentâ⬠¦Instead, it is important that the subject is approached with a sense of openness for the skeptic and a healthy amount of caution for the readily believing. In an over-arching sense, the subject must be approached carefully and critically. Before moving further in the discussion of the continuation of dreams and visions as a means of Godââ¬â¢s continued revelation today, it would be important to define briefly what exactly dreams and visions are. Defining Dreams and Visions It may seem unnecessary to define something as common to us as dreams. Each and every person, in one degree of lucidity or another, has experienced dreams and does so on a recurring basis. In defining and understanding what exactly dreams and visions are, along with their similarities and differences, we continue to turn to Morton T. Kelsey. Kelsey notes that, while we cannot certainly understand what sleep is, we can understand that it is a specific type of consciousness, a transient interruption of the waking state. During this interruption of the waking state, there are two states that appear to take effect in the human brain, known by psychologists as the ââ¬Å"primary-process activityâ⬠and the ââ¬Å"secondary-process mentation.â⬠The primary-process activity is the time during sleep in which the sleeper experiences a single picture or figure, or even a vivid and elaborate story. It is not uncommon for the sleeper to experience this phase ofShow MoreRelatedThe Functi on Of Prayer And The Islamic Faith1430 Words à |à 6 Pagesmonotheistic faith which centers around the sovereignty of Allah, the five pillars of faith, and the sanctity of the Qurââ¬â¢an. à Christianity views Islamic prayer as an opportunity for the real God reveal the truth to Muslims. à à à à à ââ¬Å"Islam is the fastest-growing major religion in the worldâ⬠(Taylor). à In Islam, Allah is almighty (Qurââ¬â¢an Sura 67:2). à The five pillars of faith, Shahada, Salat, Zakat, Hajj and Sawm, are a constant reminder of a Muslimââ¬â¢s devotion to Allah. à The five pillars are essentialRead MoreSwami Vivekananda14669 Words à |à 59 Pageshere, only in a more clear sense. Narendra was astounded and puzzled. He could feel the mans words were honest and uttered from depths of experience. He started visiting Ramakrishna frequently. Though Narendra could not accept Ramakrishna and his visions, he could not neglect him. It had always been in Narendras nature to test something thoroughly before he could accept it. He tested Ramakrishna to the maximum, but the master was patient, forgiving, humorous and full of love. He never asked NarendraRead MoreProject Managment Case Studies214937 Words à |à 860 Pagesyears, primarily due to the advanced technology developed by their Eton Division, which produces disk drives. Continental is considered one of the giants in computer technology development, and supplies equipment to other computer manufacturers. World headquarters for CCC is in Concord, Illinois, a large suburb northwest of Chicago in the heart of Illinois s technology center. In addition to corporate headquarters, there are three other divisions: the Eton Division, which manufactures disk drives
Monday, December 23, 2019
Technical And Business Of Entrepreneurship - 869 Words
â⬠¢ Technical and Business Undergraduatesââ¬Å¸ Self-Efficacy in Entrepreneurship This paper investigated how particular and business student see their ability to adjust, perform and be viable in big business. Past investigation revelations suggest that entrepreneurial objectives and exercises are impacted by individuals self-respect or self-efficacy identifying with the confidence and saw competency in business issues. Seen wellness much of the time implies data, capacities and conduct, which are considered as the priority of confidence. In that limit, business understudies may be acknowledged to have higher self-respect in business endeavors than non-business students. Nineteen (31%) of the business student and 15 (29%) of the particular student had either a relative or a relative who was a specialist. Fifty-two (84%) of the business students whilst 40 (78%) of the particular student have had the mean to get required in business with most of them wanted to do all things considered inside 5 years after Bachelorââ¬â¢s degree. The stark qualification between both social events of individuals was around 17 (27%) of the business understudies diverged from only 4 (8%) of the specific student had the mean to meander into business practices rapidly after graduation. Preliminary examinations were performed to ensure no encroachment of assumptions of normality, linearity, and homoscedasticity. â⬠¢ Self-efficacy, self-esteem, and entrepreneurship among the unemployed This study focuses onShow MoreRelatedBusiness Skills As Entrepreneurship Education1203 Words à |à 5 Pageshave explained business skills as entrepreneurship education to empower knowledge of the entrepreneurs. This entails business management skills, business planning, financial management, awareness of legal aspects and market searching. That being the case, the skills are useful and important in developing creative thinking, negotiations, leadership (business management), exposure to technical innovation and new product development. However, the skills help to identify and exploit business opportunitiesRead MoreInternational Entrepreneurship at Infusion1134 Words à |à 5 PagesInternal Entrepreneurship at Infusion Grand Canyon University LDR 640: Leadership and Innovation Michael L Verne February 29, 2012 Page 2 International Entrepreneurship at Infusion We will be examining the growth and development of Infusion, a company that has grown in the last 10 years to become a $50 million a year international software services business with over 350 employees. The vision of Infusion states ââ¬Å"Achieve global growth by measurablyRead MoreDifferent Types of Entrepreneurship1001 Words à |à 4 PagesIntroduction Entrepreneurship can simply be defined as the art and process that ââ¬Å" involves capturing ideas, converting them into products and, or services and then building a venture to take the product to marketâ⬠(Zhao, pp. 26). The essential aims to be accomplished by these procedures is the creation of a new organizations or reganerating any part of any organizations for the purpose of achieving further improvement and betterment of the comprehensive business or company. For entrepreneurs sansRead MoreSelf Efficacy Among Students From A University Or School1608 Words à |à 7 PagesThe aim of this research was to identify the level of entrepreneurial self-efficacy among students from a university or school which adopted entrepreneurial education also how entrepreneurial self-efficacy is related to gender. Self-efficacy in entrepreneurship must be measure by quantitative method. Research have come a long progress to identifying individual characteristics and skills for predicting e ntrepreneurial performance and achievement. Introduction A person belief their ability to successfulRead MoreCan Entrepreneurship Be Taught1355 Words à |à 6 Pagesâ⬠¢ Can we teach entrepreneurship and bring some entrepreneurial awareness and changes among individuals. Give evidence Approach in answering the Question â⬠¢ Definition of Entrepreneurship â⬠¢ Entrepreneurship education â⬠¢ Approaches to teach entrepreneurship â⬠¢ Conclusion The concept of entrepreneurship There is no universal agreed definition of entrepreneurship. However, Entrepreneurship is defined differently by different scholars. Schumpeter (1934) defines Entrepreneurship as a personââ¬â¢s abilityRead MoreThe Academy Of Business And Entrepreneurship Charter School1408 Words à |à 6 PagesI. SCHOOL DESIGN The Academy of Business and Entrepreneurship Charter School is a K-12 model school utilizing a unique combination of PA Common Core Standards aligned curriculum and specialized programming with a focus on developing 21st Century skills to prepare students for their post- secondary goals. It will open its doors in 2014, starting with 240 students in grades K-5 and add a grade a year to reach K-9 during the course of the initial charter. However, as the model is envisioned as a fullRead MoreEntrepreneurship Education Of Chemical Engineering1591 Words à |à 7 PagesEntrepreneurship Education in Chemical Engineering Economic trends and rapidly changing hiring conventions are fueling a rapid expansion in value awareness of entrepreneurship education to engineering students. Each year, a growing proportion of the two hundred thousand engineering graduates find work in small businesses or start-up ventures, eliciting a new type of engineer, an entrepreneurial engineer, who needs a broad range of skills and knowledge above and beyond a strong science and engineeringRead MoreThe Promise of Entrepreneurship as a Field of Research638 Words à |à 3 PagesPROMISE OF ENTREPRENEURSHIP AS A FIELD OF RESEARCH 1.0 Introduction To date, the phenomenon of entrepreneurship has lacked a conceptual framework. A research has been conducted in the difference social science disciplines and applied fields of business to create a conceptual framework for entrepreneurship. 2.0 Definition of Entrepreneurship Entrepreneurship is defined solely by the researchers in terms of who the entrepreneur is and what he or she does. The study of entrepreneurship must involveRead MoreRelationship Between Entrepreneurship And The Economic Development Essay1091 Words à |à 5 PagesBUSINESS RESEARCH METHODS Introduction This research seeks to establish the relationship between entrepreneurship and the economic development in Saudi Arabia. Entrepreneurship is the process of managing, organizing and developing a business venture in a competitive environment that is dynamic (Kuratko 2016 pp3). An entrepreneur is an inventor, leader, innovator, and pioneer and most significantly, a performer. The heart of a national advantage is often pegged on entrepreneurship. The operationRead MoreEntrepreneurship Risk, Rewards, And Reality1456 Words à |à 6 Pagesgone because of entrepreneurship. In the article ââ¬Å"Entrepreneurship Risk, Rewards, and Realityâ⬠by Timothy W. Scales, the author goes over key points about small business like development, growth, and secession. Scales focus on experiences that relate to an accomplished and an unaccomplished entrepreneur. Scales say that if an idea can become entrepreneurial, it can be big or small. Entrepreneurship is the key to innovation and growth. Since t he 1970s, scholars knew that entrepreneurship was the source
Sunday, December 15, 2019
Gay and Lesbian Advertising Free Essays
string(36) " belonging is to the gay community\." Advertising ââ¬Å"Outâ⬠of the Box In 1994, Ikea created a wave of controversy when they became one of the first companies to market to the gay community on mainstream daytime television. Despite the initial backlash; this ad eventually sparked countless other companies jump on the ââ¬Å"Gay and Lesbian Advertising Bandwagonâ⬠. But what is the motive behind advertising specifically to this community, do the businesses take a genuine interest in the gay community or is it strictly for expanding business? Additionally, once a business decides to advertise using gay content, what is the appropriate way to go about doing so, and how does their strategy differ between gays and lesbians? Only 4 to 10% of the population identifies themselves as gay or lesbian. We will write a custom essay sample on Gay and Lesbian Advertising or any similar topic only for you Order Now (Okenfull, 50) So why are businesses so concerned with gaining the support of this demographic? One of the biggest reasons being their buying power. While they donââ¬â¢t make more than heterosexual couples, a large majority are not raising children, so they have more disposable income. Thus, they have even been referred to as the ââ¬Å"Dream Marketâ⬠with a potential buying power of $641 billion annually (Okenfull, 49). Because this is the foremost reason why advertisers go after the gay community, this makes us question whether they have a genuine interest in the gay community. ââ¬Å"We market to gays and lesbians for business reasons because we want to sell out product to consumers. It doesnââ¬â¢t get more complicated than that. ââ¬Å" -Miller beer spokeswoman (Sender, 2) It is common knowledge that the only point of advertisement is to drive in more business. However, analyzing the overall impact of advertising on society, it seems as if there is more that comes into play than just a business decision. In fact, regardless of the companyââ¬â¢s motive to produce advertisements that have gay content, the overall impact of infiltrating gay content into mainstream media has actually helped advance and liberate the gay community. Big corporations not only reflect societyââ¬â¢s current values, but they are also largely responsible for shaping societyââ¬â¢s values. If there are more gay and lesbian imagery in the media, society will adapt he perception that they are everywhere and normalizes gayness. Also, having large corporations back a minority-based cause, will make the majority more accepting and make the people in opposition to the cause seem more ignorant. Essentially, even just the facade of a company publicly declaring their support for gayness makes a powerful statement to society. Not only is appearing to be in support of gay rights is something that will boost your business, but to have any anti-gay statement or claims can detrimentally hurt your company as well. In 1977, Coors was accused of firing gays, along with several other minority groups. Journal of Community Research) Not long after much controversy, chairman Pete Coors adopted an Gay-Friendly policy and also extended benefits to same-sex couples. In 2000, Coors hiked up their spending on gay advertising, putting them in the number two spot for most money spent on gay advertisements. (Chura, 1) After a company becomes seen as anti-gay, it is evident that they must fight desperately to overcome this stigma just to stay in the game. Beer is a prime example of a product thatââ¬â¢s advertisements have saturated the gay market to the extent of which if you do not participate, then youââ¬â¢re stance on gay rights is questioned. There is a very positive outcome for the many companies that show their support to the gay community as well as a negative outcome for companies that donââ¬â¢t. Despite this; it still leaves many consumers skeptical of their motives. Many advertisements, in a failed attempt to show acceptance, have become the target of gay critics. The harshest of backlash of these ads are in ones in which they depict obvious and offensive gay stereotypes. It is evident that advertisers must handle these types of ads with care, depicting gay stereotypes can lead to further segregation rather than promoting equality. Gay consumers are very skeptical to how a company presents gay advertisements, but perhaps the biggest obstacle is to still keep heterosexual consumers that are turned off by or blatantly opposed to homosexuality. When Ikea first ran their ad on daytime television, there was a positive response from gay consumers. However, during this time period it still created enough controversy that outweighed the benefits of appealing to that market. This is when agencies quickly realized that limiting their advertisements to the confines of strictly gay and lesbian print media such as OUT magazine was a safer route for the time being. Although the initial reaction by heterosexuals to this first commercial was negative and controversial, this was also a time in history where the attitude around gay media was at a turning point . Gay shows became less of a niche market, and more mainstream. ââ¬Å"More recently, gay-oriented shows such as the L Word, Will and Grace, Ellen, Queer as Folk, and Queer Eye for a Straight Guy have increasingly pushed the boundaries of cultural accepatnce of homosexuality and have diminished the risk of backlash for firms who are percieved to be ââ¬Å"gay friendly. â⬠(Okenfull, 50) Shows dealing with this content presumably have made the hetereosexual community more understanding to this type of lifestyle and generationally speaking, the current generation became much more likely to support the gay rights movement. Thus, integrating gay ads into mainstream television and print media was back on the table. Because the decision for advertisers to target the gay community is still a very controversial subject for both straight and gay consumers, it is imperative to acknowledge the vast differences in how to effectively target this audience. First, it is important to recognize gay male consumers and lesbian female consumers as a very different audience. Additionally, it is important to assess how strong their gender identity is. The strength of oneââ¬â¢s gender identity is based on several different factors, such as how strong their involvement and sense of belonging is to the gay community. You read "Gay and Lesbian Advertising" in category "Essay examples" Gay males that are identified as having a high degree of sexual identity, are more attracted to ads that display explicit gay content. (Okenfull, 54) To illustrate an ad that is ââ¬Å"explicitlyâ⬠gay, (refer to picture 1), I chose Virgin Mobiles ââ¬Å"Hook up Fearlesslyâ⬠. The ad almost positively insinuates that the two males depicted in the ad are gay, if not gay, then extremely turned on by the a gay act. This type of explicitness would be most attractive to males who have a high gay identity rather than low. There is a large power construct in this advertisement. The male with wings is forcefully holding down the other male to kiss him while the male on bottom, while he does seem submissive, he is still enjoying the act taking place. I personally like this ad; mostly because of the actual relevance to the buying process. Consumers always have an apprehension to buying products, let alone a phone contract. This ad is conveying to the potential consumer to just give in and try it. While the male on bottom might have been apprehensive about the kiss, just as ââ¬Å"Youââ¬â¢ll Love Usâ⬠guarantee gives you the freedom to chose without ââ¬Å"strings attachedâ⬠. It is a ââ¬Å"Just try it! â⬠ad, and while hooking up in an office space is taboo; doing it with with a gay angel is even more controversial and daring, and you might just ââ¬Å"Love Itâ⬠. I like this ad because it communicates the point fluidly, and replaces any uncertainty with a sense of empowerment and fearlessness. While ads like these are very effective in communicating with the demographic who have a strong sense of gender identity, low identity gays and lesbians are more responsive to a less explicit type of advertising. Generally, gays and lesbians who have a low sense of gender identity want to define themselves by traits other than just their sexual preference. By being less explicit, they get the opportunity to read between the lines, not feel alienated by their gender identity, and not have their identity to be oversexualized. To target this audience, advertisers indicates gayness without actually using a lesbian or gay couple. Instead they use implicit gay imagery which uses symbols and phrases that indicates gayness such as rainbows, pride, and being ââ¬Å"outâ⬠. (Okenfull, 55) To illustrate an add that does this, I chose Chevyââ¬â¢s electric car ad (refer to ad 2). There are a mother and father car facing their child car. The caption says ââ¬Å"Mom, Dad, Iââ¬â¢m electric. â⬠The bottom of the page uses a thin bar that is intended to look like a rainbow. The copy at the bottom is ââ¬Å"So, whatever revs your engine, we support you 100%, Happy Motor City Pride from the entire Chevrolet family. â⬠This is another ad that I think is done beautifully. They used a social norm of what society knows as the ââ¬Å"coming out sceneâ⬠. The electric car is declaring that it is not just any old car, just as a gay or lesbian would have to come out to their parents that they are not just another heterosexual. What also intrigues me about that ad is the italics on ââ¬Å"Iââ¬â¢m electricâ⬠. This is presumably a play off the upbeat ââ¬Å"Itââ¬â¢s electricâ⬠song. The car is excited to tell its parents that he is gay, conveying a sense of empowerment and excitement, instead of how we typically think of this moment, which can often be a shameful and intimidating moment for many children. I also really like the ad for itââ¬â¢s relevance to the gay community. According to a recent survey, only ? of hetereosexuals admitted to being concerned about the environment while 55% of gays and lesbians identified that they are more likely to ââ¬Å"go greenâ⬠. (Koretzky). It is targeting consumers that are interested in their message, while also encouraging acceptance and becoming empowered. While even high identity lesbians are most attracted to implicit gay imagery, their liking towards explicit lesbian imagery comes secondary (before explicit gay imagery). But interestingly enough, lesbian imagery is extremely underrepresented. It was reported that in one of leading gay and lesbian magazines, The Advocate, lesbian-targeted imagery accounted for only 3% of the magazine advertising. Why is this? (Okenfull, 65) Not only do lesbians have typically just as much buying power as gay males, but heterosexual consumers are even more comfortable with lesbian imagery over gay imagery, which lowers the risk of heterosexuals being offended or turned off from a product. In general, lesbians are less discriminated than gays. his is something that shows up in the workplace; according to a recent study, gay males make 23% less than straight males, lesbians make about the same as straight women. (Williams Institute) Perhaps society taking a particular favoritism to lesbianism is the very reason that advertisements that depicting lesbians couples in ads targeted towards lesbians are more underrepresented. There is a oversexualization attenuated by the het erosexual community of how lesbians are viewed. I chose an ad that was apart of a campaign for a swiss Italian ski resort (picture 3). A website was commenting on the success of this ad with the title of the Article ââ¬Å"Lesbians Good for Ski Business. â⬠the comment on this ad stated, ââ¬Å"Seems some brilliant ad agency came up with a campaign to promote the Swiss Italian ski resort Airolo by featuring two women about to kiss (pictured above). Weââ¬â¢re going to hazard a guess that the intended demographic was not lesbians. They were probably targeting lager louts looking for a good stag party getaway now that Prague has kicked them out. â⬠(Get Outdoor Blog) The imagery does not have any clever tagline, or anything to empower lesbains. In fact, one of the main centerpieces of the point of this ad is the male in the background completely enamored and excited by the thought of two girls kissing. The comment on the article even states that ââ¬Å"the intended demographic was not lesbains. â⬠and makes the point that this ad was to promote a party scene. In fact, most viewers probably would not even assume the two girls in the ad as lesbians. By no means does the ad seem to support the gay community, it rather just sexualizes the fantasy of watching two women make out. A sexual lesbian experience can be arousing to straight men and women, conveying a ââ¬Å"partyâ⬠type of experience while if the couple was actually a gay couple about to engage in a kiss, this ad would most likely be only arousing to gay men, and convey the message that ââ¬Å"We are a gay friendly placeâ⬠rather than a ââ¬Å"Party Placeâ⬠. Another advertisement that depicts the sexual objectification of women is the Nikon ad. There is a hand holding up a camera in which he captures the image of two women in lingerie on top of each other. While the gender of the person taking the photo is not identified, it appears to be a male. Because of the interest in the (presumed) male figure, this ad makes it very evident that the relationship between the two women is not a committed lesbian relationship. It is portrayed to be just a sexual one with an objective to please the male. The copy at the bottom states that the Nikon S60 detects up to 12 faces. When you look closer at the ad, you can spot four males in the adjacent apartment building watching the girl-on-girl action. Many consumers were very upset with this ad, believing it to be both sexist and delegitimizing to lesbian relationships. They made the argument that, the advertisement is sexist relies heavily on the notion that the lesbians are unwitting, innocent subjects violated by a voyeuristic male glaze. â⬠(Turnbell) The writer refutes this argument by stating that the two women made the conscious decision to objectify themselves by leaving the curtains open, as well letting another male to capture their (not-so) intimate moment on a camera. Addition ally, the ad is relevant to the product because it successfully communicates in a clever way that that even though the faces are so far away, the camera is advanced enough to still detect the voyeurs faces. Personally, I completely agree with the writerââ¬â¢s argument. However, I would also argue that these types of ads do present a problem to the lesbian community. How can advertisements tastefully depict a lesbian encounter that meaningfully supports and empowers the lesbian community? Also, how can you conveys that the two women are legitimately homosexual, rather than a sexualizing their experience? This objectification of the lesbian relationship is likely why many lesbian advertisements are underrepresented. In general, they are most comfortable to advertisements that use implicit hints of sexuality rather than involving males or females (such as the car commercial). Some argue that ââ¬Å"lesbians are female versions of male sexualityâ⬠, but this argument is scrutinized because lesbians describe their experience to be completely different. Typically, lesbians are more socially accepted than gay males are. However, they have to fight harder to convince society that their identity is more than just a sexual desirability of a female. One company that has successfully done so is JC Pennyââ¬â¢s. In 2012, they released their ââ¬Å"Freedom of Expressionâ⬠ad (See Picture 5) that features two mothers and their daughter. While a group called ââ¬Å"One Million Mothersâ⬠presented backlash to this ad, the overall response was positive. There is no overt sexual nature in this picture, it is just depicting a family photo with two mothers. ââ¬Å"As jcpenney focuses on becoming Americaââ¬â¢s favorite store, we want to be a store for all Americans. In celebration of Motherââ¬â¢s Day, weââ¬â¢re proud that our May book honors women from diverse backgrounds who all share the heartwarming experience of motherhood. â⬠-Eric Bovin (ABC news) In conclusion; advertisers have recognized the great importance of tapping into the gay market. Not only is establishing your company as being in support of this cause positive, but in some markets; it is necessary. Overall, Advertising to the gay and lesbian community is not one-size-fits-all. It are also specific precautions that you must take in entering into this type of market. Not only are you going up against the criticisms of anti-gay groups, but you are also up against the scrutiny of whether your advertisement is legitimate or whether you are using the leverage of the gay community to seem more accepting and grow your consumer base. There are Advertising Agencies must take into account the way in which homosexuals identify themselves in terms of their involvement as well as the how males and females differ in what appeals to them. Works Cited Hilary Chura, ââ¬Å"Coors hikes spending on gay ads, March 27, 2000 Katherine Sender, Business Not Politics: The Making of the Gay Market, New York: Columbia University Press, 2005, 331 pp. , ââ¬Å"The Dynamics of Brand Legitimacy: An Interpretive Study in the Gay Menââ¬â¢s Community (PDF)â⬠. Journal Article, Journal of Consumer Research, University of Chicago Press. JSTOR 10. Going Green, Going Gay? Micheal Koretzky, Jan 13, 2011 The Williams Institute, ââ¬Å"Documented Evidence of Employment Discrimination and Its Effects on LGBT Peopleâ⬠(July 2011). Get Outdoors Blog, ââ¬Å"Lesbians for Good Ski Businessâ⬠(Decemebr 21,2007) James Turnbell, ââ¬Å"A Sexist Advertisement? Lesbians and the Politics of the Male Glazeâ⬠(December 13, 2008) ABC News, ââ¬Å"JC Penney Features Same Sex Couple in May Catalougeâ⬠, (May 12, 2012) How to cite Gay and Lesbian Advertising, Essay examples
Friday, December 6, 2019
Medicinal Chemistry
Questions: 1. Consider, for example, conducting a microbiological assay where inhibition of growth of bacteria is determined when different concentrations of drugs are applied. What factors may lead to variability or errors within the results and how could these variations or errors be minimised?2. Why is it important to have information on physico-chemical properties (such as logarithm of the octanol:water partition coefficient (log P) and log aqueous solubility (log S)) in drug development i.e. what role do these properties play in determining formulation and drug uptake / distribution in the body?3. Is predicting ADME and potential toxicity of drug candidates early in the drug development process important or should the focus of research be to maximise efficacy of the candidate molecules? Your answer should include a discussion of the advantages and limitations of using simple screening tools, such as Total polar surface area (TPSA), violations for Lipinskis Rule of Fives and number of rotat able bonds may be used in screening and normalised occurrence ratios (NORs), potential toxicity and metabolism? Answer: 1. Microbiological assays are mostly conducted in the laboratory to check the bacterial growth inhibition in the presence of different drug concentration. This can be performed in a plates containing growth medium. Zone of equivalence is found within the plates, which represents the concentration at which a particular microbial growth is inhibited by particular drug (Zuccheri and Asproulis, 2012). Various factors may affect the results. These are improper preparation of growth medium, improper selection of the population number, imbalanced moisture, pH, nutrient content of the growth medium. These errors can be minimized by maintaining proper sterile condition during growth medium preparation, correct selection of drug concentration. 2. LogP and logs coefficients are distinguished as the most important parameters for lipophilicity assessment, estimation of chemical compounds solubility and determination of pharmacokinetic properties. Tendency of molecules to get dissolve in lipids is generally calculated as logP. Tendency of molecules to get dissolve in water lipids is generally calculated as logS (Sun, 2004). So, when a drug molecule is developed, it is very much essential to understand the basic property of that particular molecule, whether that molecule will be taken up by the human system and distributed properly within a certain period of time, hence quantification of logP and logs is very much significant. 3. Prediction of absorption, distribution, metabolism and excretion and potential drug toxicity initially during the drug development process is important and the related research focus should be made to maximize the effectiveness of the candidate molecule. This is because it helps in predicting enzyme inhibition, such as: CYP3A4 Noninhibitor, CYP2D6 Noninhibitor, CYP2C19 Noninhibitor, CYP2C9 Noninhibitor, CYP1A2 Inhibitor. Fluvoxamine is a strong CYP1A2 Inhibitor and inhibits some of the cytochrome P450 enzymes, like: CYP3A4, CYP2C9 etc. Thus, fluvoxamine prevents few metabolic pathways from recompensing for CYP1A2 inhibition. This type of enzymatic inhibition or non-inhibition can be predicted with the help of screening tools, which is considered as advantage. Disadvantages may include unrestrained adsorption methods or matrix trapping or covalent bound or immobilization methods, low quantity enzymatic activity along with the limitation of mass transfer. ADME properties include abs orption, distribution, metabolism and excretion and potential drug toxicity and these properties determine the drug disposition once a drug enters into the system. Total polar surface area or TPSA of a candidate molecule is identified as the sum of the surface over polar atoms, for example: nitrogen, oxygen, including attached hydrogens (Chatwal, 2010). TPSA is used to optimize the ability of a candidate drug to permeate cells. Molecules with more than 140 squared are likely to be poor at cell membrane permeation. Lipinskis rule of five evaluates drug-likeliness and find out whether a chemical compound with particular biological or pharmacological activity has characteristic properties, which would make the compound an expected orally active medicine in humans. Mostly orally consumed drugs are comparatively small and lipophilic molecules. The advantage of Lipinskis rule of five is that it illustrates the significance of certain molecular properties for a particular drug pharmacokine tics inside the human system. This includes absorption, distribution, metabolism and excretion. The major disadvantage of this rule is that it does not calculate whether the candidate molecule is active pharmacologically. To predict a candidate molecule, the atomic environment in the candidate molecule is calculated and then database is searched for comparable surroundings. Occurrence ratio measures how often the similar surrounding has been discovered, compared with how frequently it has been found out in total, and then is calculated for individual atom in the candidate molecule (Carlsson et al., 2010). This ratio present comparative probability of metabolism taking place at individual atom but the disadvantage is that it does not predict whether the candidate molecule undergoes metabolism. References Carlsson, L., Spjuth, O., Adams, S., Glen, R. and Boyer, S. (2010). Use of historic metabolic biotransformation data as a means of anticipating metabolic sites using MetaPrint2D and Bioclipse.BMC Bioinformatics, 11(1), p.362. Chatwal, G. (2010).Medicinal chemistry. Mumbai [India]: Himalaya Pub. House. Sun, H. (2004). A Universal Molecular Descriptor System for Prediction of LogP, LogS, LogBB, and Absorption.Journal of Chemical Information and Modeling, 44(2), pp.748-757. Zuccheri, G. and Asproulis, N. (2012).Detection of pathogens in water using micro and nano-technology. London: IWA Publishing.
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